Tris | Solubility of Things

Identification

Molecular formula

C₄H₁₁NO₃

CAS number

77-86-1

IUPAC name

2-amino-2-(hydroxymethyl)propane-1,3-diol

State

Tris is a solid at room temperature and is usually found in its crystalline form.

Melting point (Celsius)

172.00

Melting point (Kelvin)

445.00

Boiling point (Celsius)

219.85

Boiling point (Kelvin)

493.00

General information

Molecular weight

121.14g/mol

Molar mass

121.1360g/mol

Density

1.5120g/cm³

Appearence

Tris is a white crystalline solid. It is odorless and hygroscopic, which means it tends to absorb moisture from the air.

Comment on solubility

Solubility of 2-amino-2-(hydroxymethyl)propane-1,3-diol

2-amino-2-(hydroxymethyl)propane-1,3-diol, commonly known as tris(hydroxymethyl)aminomethane or simply Tris, exhibits notable solubility characteristics in various solvents:

Water: Tris is highly soluble in water, making it a widely used buffering agent in biological and chemical applications. The hydroxymethyl groups enhance hydrogen bonding, facilitating its dissolution.
Organic Solvents: While Tris is primarily water-soluble, it shows limited solubility in organic solvents like ethanol and acetone, due to its polar character.
pH Dependence: The solubility can be influenced by pH levels, as Tris is a zwitterion at physiological pH values, promoting its solubility in aqueous solutions.

In practice, you may observe:

Tris solutions are commonly prepared at concentrations ranging from a few mM to several hundred mM.
Due to its high solubility, it is essential to avoid concentrated solutions, which can lead to precipitation under certain conditions.
Tris is often used to create stable buffering systems in laboratory settings due to its ability to maintain pH without significantly precipitating out of solution.

In summary, the solubility of 2-amino-2-(hydroxymethyl)propane-1,3-diol is a key feature that underpins its utility in various scientific disciplines. Its high solubility in water and specific behavior in different solvent systems underscore its importance as a versatile reagent.

Interesting facts

Interesting Facts about 2-Amino-2-(hydroxymethyl)propane-1,3-diol

2-Amino-2-(hydroxymethyl)propane-1,3-diol, commonly known as tris, is a fascinating compound with diverse applications in chemistry and biochemistry. Here are some intriguing aspects of this valuable molecule:

Buffering Agent: Tris is widely used in biological and biochemical research as a buffering agent. It helps maintain a stable pH in various laboratory environments, which is crucial for enzyme activity and other biological processes.
Biocompatibility: Due to its low toxicity and biocompatible nature, Tris is often used in cell culture, providing an optimal environment for cellular growth and proliferation.
Role in DNA and RNA Research: Tris is essential in molecular biology applications, particularly in the preparation of buffer solutions for techniques such as gel electrophoresis. Its ability to stabilize nucleic acids makes it invaluable in laboratory settings.
Tris Buffer Systems: The versatility of Tris allows it to be combined with other compounds to create a range of buffer systems with varying pH levels. Typical pH ranges for Tris buffers are between 7 and 9, making it suitable for various biological assays.
Citation in Publications: The compound has been mentioned in numerous scholarly articles and research studies, demonstrating the integral role it plays in biochemical experiments. As noted by many researchers, "without Tris, modern molecular biology would not be where it is today."

Overall, 2-amino-2-(hydroxymethyl)propane-1,3-diol stands out as a crucial compound in the scientific field, emphasizing the importance of buffers in maintaining physiological conditions and facilitating experiments. Its widespread usage and utility make it a significant player in the advancement of biochemical research.

Synonyms

Trometamol

TROMETHAMINE

77-86-1

Tris(Hydroxymethyl)aminomethane

Tris

Tham

2-Amino-2-(hydroxymethyl)propane-1,3-diol

Trisamine

Trizma

2-Amino-2-(hydroxymethyl)-1,3-propanediol

Tris buffer

Tris base

Tromethane

Tris-base

Trisaminol

Pehanorm

Talatrol

Trisamin

Trispuffer

Tutofusin tris

Addex-tham

Tris-steril

1,3-Propanediol, 2-amino-2-(hydroxymethyl)-

Tris, free base

Trimethylolaminomethane

Aminotrimethylolmethane

Aminotris(hydroxymethyl)methane

Tromethanmin

Tris (buffering agent)

Tris Amino

Tris(hydroxymethyl)methanamine

Tris(hydroxymethyl)methylamine

THAM-E

Tromethamolum

Trometamole

Trizma base

Trometamolum

2-Amino-2-methylol-1,3-propanediol

Caswell No. 036

Tris-hydroxymethylaminomethane

bakerbond(tm) cyano (cn)

NSC 6365

Methylamine, 1,1,1-tris(hydroxymethyl)-

2-(Hydroxymethyl)-2-amino-1,3-propanediol

ABX (ANTIBODY EXCHANGER)

HSDB 3408

Tris-Amino

Methanamine, 1,1,1-tris(hydroxymethyl)-

Trometamolum [INN-Latin]

NSC-6365

EINECS 201-064-4

UNII-023C2WHX2V

MFCD00004679

Trometamol [INN]

Tris-hydroxymethyl-aminomethan

EPA Pesticide Chemical Code 083901

023C2WHX2V

CHEBI:9754

WP QUAT (STRONG ANION EXCHANGER)

2-Amino-2-hydroxymethyl-1,3-propanediol

DTXSID2023723

Tris(hydroxymethyly)amino methane

AI3-03948

NSC6365

126850-03-1

126850-06-4

126850-08-6

Tromethamine [USAN:USP]

AMINO (NH2) NARROW-PORE MEDIA-NORMAL PHASE

DTXCID503723

tris(hydroxymethyl) aminomethane

126850-05-3

Trometamol(Tris),Proteomics Grade

EC 201-064-4

NSC65434

THAM-E COMPONENT TROMETHAMINE

1,1,1-tris(hydroxymethyl)methanamine

Trometamol(Tris),for molecular biology

NCGC00159412-02

NCGC00159412-04

Tris buffertris-hydroxymethyl-aminomethan

TROMETHAMINE (II)

TROMETHAMINE [II]

Trometamolum (INN-Latin)

Tromethamine (USAN:USP)

TROMETAMOL (MART.)

TROMETAMOL [MART.]

WLN: Q1XZ1Q1Q

136760-04-8

Trometamol(Tris), inverted exclamation markY99.5%

TROMETHAMINE (USP-RS)

TROMETHAMINE [USP-RS]

4-Anilino-1-(2-hydroxyethylamino)anthracene-9,11-dione

Tri(hydroxymethyl)aminomethane

Tromethamine [USAN]

(tris(hydroxymethyl)aminomethane)

[tris(hydroxymethyl)aminomethane]

TROMETAMOL (EP MONOGRAPH)

TROMETAMOL [EP MONOGRAPH]

1, 2-amino-2-(hydroxymethyl)-

Triladyl

Trigmo base

TROMETHAMINE (USP MONOGRAPH)

TROMETHAMINE [USP MONOGRAPH]

MFCD00132476

TRIS(HYDROXY-D-METHYL)AMINO-D2-METHANE, 98 ATOM % D

Methanamine,1,1-tris(hydroxymethyl)-

Methylamine,1,1-tris(hydroxymethyl)-

tris(hydroxymethyl)amino methane

.beta.-D-ribo-Hexopyranose, 1,6-anhydro-3-deoxy-2-O-(1-methylethyl)-4-O-(phenylmethyl)-

CAS-77-86-1

Tris(hydroxymethyl)aminomethane, >=99.8%

propane, 2-amino-1,3-dihydroxy-2-hydroxymethyl-

Tris(hydroxymethyl)aminomethane, Electrophoresis Grade

Tris-hydroxymethyl-aminomethan [German]

trometamolo

Tromethamin

Aminotri(hydroxymethyl)methane

tris-buffer

tris-amine

Tro.meta.mole

Tris(Hydroxymethyl)-Aminomethane

Tro.meta.mol

1gng

tris base solution

83147-39-1

THAM (Standard)

TRS

Tromethamine (USP)

TRIS Ultrapure, EP

1h4n

Trometamol (JAN/INN)

TROMETAMOL [JAN]

TROMETHAMINE [MI]

SCHEMBL975

trishydroxymethylmethylamine

THAM (TN)

TROMETHAMINE [HSDB]

NCIOpen2_000263

NCIOpen2_001720

TROMETAMOL [WHO-DD]

TROMETHAMINE [VANDF]

Oprea1_677781

trishydroxymethyl aminomethane

tris-hydroxymethyl-methylamine

MLS000028643

tris hydroxymethyl aminomethane

tris-(hydroxymethyl)methylamine

Tris (hydroxymethyl)aminoethane

GTPL7328

tris (hydroxymethyl)aminomethane

CHEMBL1200391

tris (hydroxymethyl) methylamine

HY-D0227C

HY-D0227D

HY-D0227E

HY-D0227R

tris (hydroxymethyl) aminomethane

B05BB03

B05XX02

Tris-(hydroxymethyl)-aminomethane

2-Amino-2-hydroxymethylpropanediol

HMS3652L05

HMS3885H09

tris-(hydroxymethyl)-amino-methane

TROMETHAMINE [ORANGE BOOK]

2-amino-2-hydroxymethyl-propane-1

Tris-Base, molecular biology grade

BCP05578

HY-D0227

STR03166

Tox21_111645

Tox21_201646

Tox21_303167

BBL000011

NSC-65434

s4176

STK379529

1,1,1-tris(hydroxymethyl)methylamine

2-amino-2-methylol-propane-1,3-diol

AKOS000121321

Tox21_111645_1

1,1,1-Tris(hydroxymethyl)-methylamine

CCG-214012

CS-W018524

DB03754

FT15751

PB47623

Trizma(R) base, >=99.0% (T)

Tris(hydroxymethyl)aminomethane, >=99%

TROMETHAMINE COMPONENT OF THAM-E

NCGC00159412-03

NCGC00159412-05

NCGC00257164-01

NCGC00259195-01

BP-13394

DA-58763

SMR000059179

Tris(hydroxymethyl)aminomethane ACS grade

Tris(hydroxymethyl)aminomethane, ultrapure

2-amino-2-[hydroxymethyl]-1,3-propandiol

DB-091324

DS-014869

Methanamine, 1, 1,1-tris(hydroxymethyl)-

Tris acidimetric, NIST(R) SRM(R) 723e

A0321

CS-0201542

CS-0201543

CS-0201544

NS00002396

SW219208-1

T2516

TRIS-buffered saline (TBS, 10X) pH 7.4

TRIS-buffered saline (TBS, 10X) pH 7.6

TRIS-buffered saline (TBS, 10X) pH 8.0

TRIS-buffered saline (TBS, 20X) pH 7.4

2-(Hydroxymethyl)-2-amino-1, 3-propanediol

2-amino-2-(hydroxyl-methyl)propane-1,3-diol

2-amino-2-(hydroxymethyl) propane-1,3-diol

2-Amino-2-(hydroxymethyl)-1,3-propanediol;

2-Amino-2-(hydroxymethyl)-propane-1,3-diol

EN300-21687

Trizma(R) base, BioUltra, >=99.8% (T)

Trometamol (part of FOSFOMYCIN TROMETAMOL)

Tromethamine, meets USP testing specifications

C07182

D00396

M02623

P17498

SBI-0653855.0001

AB00443859_03

AB00443859_04

Q413961

Sigma 7-9(R), >=99% (titration), crystalline

SR-01000944234

Trizma(R) base, puriss. p.a., >=99.7% (T)

SR-01000944234-1

Trizma(R) base, >=99.9% (titration), crystalline

Trizma(R) base, Vetec(TM) reagent grade, >=99%

BRD-K47978074-001-02-4

BRD-K47978074-001-03-2

Tris(hydroxymethyl)aminomethane, ACS reagent, 99.9%

F0001-1979

Tris(hydroxymethyl)aminomethane, ACS reagent, >=99.8%

Tris(hydroxymethyl)aminomethane, Molecular Biology Grade

TRIS-buffered saline (TBS, 10X, high salt) pH 7.4

Z104509094

Tris(hydroxymethyl)aminomethane, p.a., ACS reagent, 99.8%

Tris(hydroxymethyl)aminomethane, ultrapure grade, >=99.9%

Trizma(R) base, puriss. p.a., buffer substance, >=99.5%

Trometamol, European Pharmacopoeia (EP) Reference Standard

Tris(hydroxymethyl)aminomethane, JIS special grade, >=99.0%

TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot

InChI=1/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H

Trizma(R) base, anhydrous, free-flowing, Redi-Dri(TM), >=99.9%

Trizma(R) base, BioUltra, for molecular biology, >=99.8% (T)

Tromethamine, United States Pharmacopeia (USP) Reference Standard

Trizma(R) base, cell culture tested, >=99.9% (titration), crystalline

Trizma(R) base, BioXtra, pH 10.5-12.0 (1 M in H2O), >=99.9% (titration)

Trizma(R) base, Primary Standard and Buffer, >=99.9% (titration), crystalline

Tromethamine, Pharmaceutical Secondary Standard; Certified Reference Material

2-Amino-2-(hydroxymethyl)-1,3-propanediol;Tromethamine;Tris(hydroxmethyl)aminomethane;Trometamol

201-064-4

79261-03-3

Trizma(R) base, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, >=99.9% (titration)

Trizma(R) base, certified reference material for titrimetry, certified by BAM, according to ISO 17025, >=99.5%